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qscripttm microdna cdna synthesis kit  (Quanta Biosciences)


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    Structured Review

    Quanta Biosciences qscripttm microdna cdna synthesis kit
    Qscripttm Microdna Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qscript+flex+cdna+synthesis+kit/qScript+Flex+cDNA+Synthesis+Kit/10__1016_slash_j__omtn__2026__102927-240-8-13
    Average 96 stars, based on 289 article reviews
    qscripttm microdna cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Behavioral and epileptic phenotypes in a CHD2 ‐related developmental delay model
    Article Snippet: .. Reverse transcription was done using qScript Flex cDNA synthesis kit (Quanta Biosciences), using random primers. .. Quantitative polymerase chain reaction (PCR) was performed in a ViiA 7 Real‐Time PCR System (Thermo Fisher Scientific) in a 10‐μL reaction mixture containing .1 mmol·L −1 forward and reverse primers, fast SYBR master mix (Applied Biosystems), and template cDNA.

    Article Title: Behavioral and epileptic phenotypes in a CHD2-related developmental delay model.
    Article Snippet: 1Department of Human Molecular Genetics and Biochemistry, Gray Faculty of Medical & Health Sciences, Goldschleger Eye Research Institute, Tel Aviv University, Tel Aviv, Israel 2Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel 3Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel 4Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel

    cDNA Synthesis:

    Article Title: Behavioral and epileptic phenotypes in a CHD2 ‐related developmental delay model
    Article Snippet: .. Reverse transcription was done using qScript Flex cDNA synthesis kit (Quanta Biosciences), using random primers. .. Quantitative polymerase chain reaction (PCR) was performed in a ViiA 7 Real‐Time PCR System (Thermo Fisher Scientific) in a 10‐μL reaction mixture containing .1 mmol·L −1 forward and reverse primers, fast SYBR master mix (Applied Biosystems), and template cDNA.

    Article Title: Viral entry shapes HCMV latency establishment
    Article Snippet: .. Total RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo Research) following the manufacturer’s instructions. cDNA was prepared using the qScript FLEX cDNA Synthesis Kit (Quanta Biosciences) following the manufacturer’s instructions. qPCR was performed using SYBR Green PCR master-mix (ABI) on the QuantStudio 12K Flex (ABI). ..

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men.
    Article Snippet: .. Quantitative real time PCRs AR TI CL E IN P RE SS Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table S5). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    Article Title: SIR-2.3/SIRT4 loss enhances proteostasis and neuronal resilience via AMPK-induced autophagy in Huntington’s disease models
    Article Snippet: Total RNA of human cell cultures was extracted using RNAbee (Tel-Test Inc.). .. Complementary DNA (cDNA) was generated using qScript Flex cDNA synthesis kit (Quantabio). .. SybrGreen real-time quantitative PCR (qPCR) experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad) following the manufacturer’s instructions.

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men
    Article Snippet: .. Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table ). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology
    Article Snippet: The isolated RNA was then resuspended in DNase/RNase-free H 2 O and DNase-treated using the Baseline-ZERO DNase (Biosearch Technologies), following the manufacturer’s recommendations and incubating the RNAs for 1 hr at 37°C. .. RNA was then retrotranscribed using the qScript Flex cDNA Synthesis Kit (Quanta Bio), following standard conditions as suggested by the manufacturer. .. The resulting cDNA was finally diluted 1:5-1:10 with DNase/RNase-free H 2 O and used as input for Quantitative Real-Time PCR using the Fast SYBR Green Master Mix (Applied Biosystems).

    Article Title: Behavioral and epileptic phenotypes in a CHD2-related developmental delay model.
    Article Snippet: 1Department of Human Molecular Genetics and Biochemistry, Gray Faculty of Medical & Health Sciences, Goldschleger Eye Research Institute, Tel Aviv University, Tel Aviv, Israel 2Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel 3Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel 4Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel

    Real-time Polymerase Chain Reaction:

    Article Title: Viral entry shapes HCMV latency establishment
    Article Snippet: .. Total RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo Research) following the manufacturer’s instructions. cDNA was prepared using the qScript FLEX cDNA Synthesis Kit (Quanta Biosciences) following the manufacturer’s instructions. qPCR was performed using SYBR Green PCR master-mix (ABI) on the QuantStudio 12K Flex (ABI). ..

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men.
    Article Snippet: .. Quantitative real time PCRs AR TI CL E IN P RE SS Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table S5). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men
    Article Snippet: .. Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table ). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    SYBR Green Assay:

    Article Title: Viral entry shapes HCMV latency establishment
    Article Snippet: .. Total RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo Research) following the manufacturer’s instructions. cDNA was prepared using the qScript FLEX cDNA Synthesis Kit (Quanta Biosciences) following the manufacturer’s instructions. qPCR was performed using SYBR Green PCR master-mix (ABI) on the QuantStudio 12K Flex (ABI). ..

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men.
    Article Snippet: .. Quantitative real time PCRs AR TI CL E IN P RE SS Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table S5). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    Article Title: Conserved small RNA networks link inflammation to pain signaling in mice and men
    Article Snippet: .. Synthesis of cDNA was done using the RNA Poly(A) Tailing Kit (MCLAB, RPTK-200) and qScript Flex cDNA Synthesis Kit (Quantabio, 95049) with proprietary universal adapter, followed by qPCR using PerfeCTa® SYBR® Green FastMix® Low ROX (Quantabio, 95074) with specific forward primers and proprietary universal adapter reverse primer (Table ). .. The CFX384 Touch Real-Time PCR System (Bio-Rad) was used for quantification in 384-well plates (Bio-Rad, HSP3805) and the CFX Maestro software (Bio-Rad v4.1.2433.1219) was used for extracting Cq values.

    Polymerase Chain Reaction:

    Article Title: Viral entry shapes HCMV latency establishment
    Article Snippet: .. Total RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo Research) following the manufacturer’s instructions. cDNA was prepared using the qScript FLEX cDNA Synthesis Kit (Quanta Biosciences) following the manufacturer’s instructions. qPCR was performed using SYBR Green PCR master-mix (ABI) on the QuantStudio 12K Flex (ABI). ..

    Generated:

    Article Title: SIR-2.3/SIRT4 loss enhances proteostasis and neuronal resilience via AMPK-induced autophagy in Huntington’s disease models
    Article Snippet: Total RNA of human cell cultures was extracted using RNAbee (Tel-Test Inc.). .. Complementary DNA (cDNA) was generated using qScript Flex cDNA synthesis kit (Quantabio). .. SybrGreen real-time quantitative PCR (qPCR) experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad) following the manufacturer’s instructions.

    other:

    Article Title: Viral entry shapes HCMV latency establishment.
    Article Snippet: In parallel, 4 μL of Lipofectamine RNAiMAX (Thermo Fisher) was AR TI CL E IN P R SS ARTICLE IN PRESS diluted in 96 μL Opti-MEM to generate Solution II.



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    RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 <t>cDNA,</t> transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).
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    Image Search Results


    RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

    Journal: eLife

    Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology

    doi: 10.7554/eLife.106846

    Figure Lengend Snippet: RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

    Article Snippet: RNA was then retrotranscribed using the qScript Flex cDNA Synthesis Kit (Quanta Bio), following standard conditions as suggested by the manufacturer.

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Control, Standard Deviation